Cyanoditolyl Tetrazolium Chloride （CTC）

CTC, 產(chan) 品目錄編號： # 19292
•5-氰基-2,3-二（4-甲基苯基）四唑氯化物（Cyanoditolyl tetrazolium chloride）可用於(yu) 測定腫瘤細胞的氧化還原活性。
•其也可用於(yu) 對食品樣本、環境樣本（尤其是水樣）進行活細菌表位熒光顯微計數。
•運用該方法就可定量檢測海水中的細菌。
•該方法可在2至10分鍾內(nei) 檢測到80%的活細菌。
•當將細胞樣本與(yu) CTC混合儲(chu) 藏於(yu) 冰箱中或液氮中時，可至少保存四星期而不導致大量細胞死亡。

訂貨信息：

產(chan) 品具體(ti) 信息

Cyanoditolyl tetrazolium chloride has been used to measure the redox activity of tumor cells. It has also been employed for direct epifluorescent microscopic enumeration of respiring bacteria in food samples and environmental samples, especially water samples. Reveals a quantitative methodology for measuring marine bacteria. These methods yield 80% activity in 2 to 10 minutes. Cell samples mixed with CTC can be stored refrigerated or frozen in liquid nitrogen for at least 4 weeks without a significant loss of cells.

Micrograph: E.coli bacteria in CTC

Formazan fluoresces in solid state Exc max: 450nm Emiss max: 630nm

Molecular Weight: 311.8

Melting Point: 230°

Soluble In: ~100mg in 6.6cc water

Photo: E. coli bacteria in CTC

References: Histochem. J. 19, 21 （1987）; Appl. Environ. Microbiol., 58, 1801 （1992）; Appl. Environ. Microbiol., 65（5）, 1966 （1999）; Appl. Environ. Microbiol., 69（4）, 2166 （2003）; Flow Cytometric Analysis: Appl. Environ. Micro. June 1999, p. 2409-2417 Vol. 65 （6）

Hazards: Irritant, Unknown

Handling: Glove, chemical goggles & fume hood

Storage: Protect from light, Store at 4 degrees celsius